Rotavirus Infection of Cultured Primary Murine Small Intestinal Epithelial Cells 874

Primary murine small intestinal epithelial cells, maintained in long-term culture, are susceptible to rotavirus infection.

We describe a method for long-term culture of primary small intestinal epithelial cells (IEC) from suckling mice. IEC were digested from intestinal fragments as small intact units of epithelium (organoids) by using collagenase and dispase. IEC proliferated from organoids on a basement-membrane-coated culture surface and remained viable for 3 weeks. Cultured IEC had the morphologic and functiona...

Murine intestinal antibody response to heterologous rotavirus infection.

Rotavirus is the most important worldwide cause of severe gastroenteritis. Extensive efforts have been devoted to the design of a vaccine that will prevent disease, but development of a more effective vaccine strategy may require progress in the understanding of the mucosal immune response to replicating viral antigens. In this article, we report the characterization of the intestinal antibody ...

Chlamydia Infection in Murine Oviduct Epithelial Cells

Identifying a Role for TLR3 in the Innate Immune Response to 1 Chlamydia Infection in Murine Oviduct Epithelial Cells 2 3 Wilbert A. Derbigny†, LaTasha R. Shobe, Jasmine C. Kamran, Katherine S. Toomey, and 4 Susan Ofner 5 Department of Microbiology and Immunology, Department of Medicine, Department of 6 Biostatistics; Indiana University School of Medicine 7 Indianapolis, Indiana 46202 8 9 10 11...

Characterization of Long-Term Cultured Murine Submandibular Gland Epithelial Cells

PURPOSE Human and rat salivary gland cell lines derived from tumors or genetic modification are currently available for research. Here, we attempted to culture and characterize long-term cultured cells spontaneously derived from wild type murine submandibular glands (SGs). METHODS SGs were removed from 3-week-old C57B/6J female mice and dissociated by collagenase type 1 and hyaluronidase dige...

Pathogenesis of intestinal and systemic rotavirus infection.